After the resignation of two officers of Auto-Train Corp. the Securities and Exchange Commission issued new subpoenas for records of the Washington railroad.

The additional subpoenas came after the resignation of Seymour Kleinman, Auto-Train's secretary, general counsel and a member of the board of directors, and Allan Menell, vice president for finance and treasurer.

Auto-Train executives said the resignations are not related to the investigation by the SEC of the company which carriers passengers and their cars between Lorton, Va., and Sanford, Fla.

Menell said yesterday he has not been questioned by the SEC, and Kleinman could not be reached for comment.

Other former employes of Auto-Train, however, confirmed that they have been quizzed about a wide variety of company actions.

When he resigned in January, Menell said it was because of "differences of management philosophy" with Eugene K. Garfield, the company's founder, chairman and president. Menell was the latest of more than half a dozen top aides to Garfield who have left the firm in the last few years.

There is one public link between the resignations of the company's treasurer and secretary. A cousin of Menell, Norman Menell is an attorney in the New York law firm of Golenbock and Barell, of which Kleinman is also a member.

Kleinman was a veteran member of the Auto-Train board, serving since the company first sold stock to the public in 1971.

Activities that took place about the time the company was being started are reported to be one focus of the SEC investigation.

The investigation had been underway for several months and was described as "a fishing expedition" by Auto-Train executives.

Among the topics said to be under investigation are reports of illegal use of corporate funds, including alleged political contributions.

Garfield has been politically active for both Democratic and Republican candidates. In 1972 he announced that he would head a fund raising committee for Sen. Vance Hartke (D-Ind.), then withdrew from the post.

In the 1976 election, Garfield supported Gerald R. Ford, hosting a fund raiser for Ford at his Washington home. Garfield has flatly denied reports that Auto-Train employes helped arrange the party and Auto-Train funds paid for it.