Dr. Charlie N. Barron, 53, a veterinary pathologist, died of cancer last Friday at the National Institutes of Health Clinical Center.

He had been senior veterinary pathologist with Tracor Jitco, Inc., in Rockville since 1975. He lived in Mount Airy, Md.

Born in Bryan, Tex., Dr. Barron received his doctor of veterinary medicine and master's degrees from Texas A & M University and a doctorate in pathology from the University of Michigan.

He had served on the faculities of Texas A & M and Oklahoma State University.

Dr. Barron also had served with the Army and the Air Force. He twice had been assistant chief of the veterinary pathology section of the Armed Forces Institute in the 1950s.

In 1959, he became assistant chief of the pathology and toxicology section of Smith Kline and French Laboratories in Philadelphia, where he served for 11 years, and was director of pathology at the Flow Laboratories, Inc., in Rockville.

Dr. Barron had been editor of the journal, "Pathologia Veterinaria," and was the author and coauthor of numerous scientific papers.

He was a member of the Society of Pharmacological and Environmental Pathologists and the American Veterinary Medical Association and a diplomate and past president of the American College of Veterinary pathologists.

He is survived by his wife, Wanda, and a son, Charlie N. II, of the home; two daughters, Ann Matheson, of Florida, and Lynn Aldrich, of California, and two grandchildren.

The family suggests that expressions of sympathy be in the form of contributions to the American Cancer Society.