Timberlake Shirley McCue, 58, a real estate developer in Northern Virginia, died Tuesday at Oak Meadow Nursing Home in Alexandria after a long illness.

Born in Charlottesville, he was a graduate of the University of Virginia. Mr. McCue moved to the Washington area in 1937, and established himself as a real estate developer with offices in Alexandria.

He helped develop Timberlake Estates and Dale View Manor in Fairfax County, and built a number of individual homes. In addition, he helped develop the Merrilee Industrial Park in Fairfax Coaunty, as well as other industrial parks.

He remained active in the business until suffering a stroke about 1 1/2 years ago. He had been a resident for many years of Belle Haven.

Mr. McCue was president of the Fairfax County Chamber of Commerce in 1964. He also had served as vice president and head of the transportation committee and played a key role in promoting the rapid mass transportation needs of the county and Dulles Airport.

He is survived by a son, Timberlake S. Jr., and a daughter, Martha Elise, of Stratford-on-the-Potomac; three sisters, Mrs. Nesbit Carwile and Mrs. Carson Fifer, both of Alexandria, and Kathleen VanEpps, of Silver Spring; a brother, Leslie, of Lynchburg, and a grandchild.