It's pay-up time for mayor-elect Marion Barry.

One by one, those in the self-described motley crew that propelled Barry to victory are calling in their political IOU's from the next mayor of the District of Columbia.

Gay rights advocates, whose early support for Barry in the cold and lonely underdog days of March was lauded by Barry as the "New Hampshire of D.C.," have submitted a 32-point "gay agenda" for the new administration. It includes among other things, a request that gay men and lesbians be appointed to a city board or commission.

A coalition of feminist groups has given Barry a list of 30 females it considers qualified for top appointive jobs in the new government. The coalition has indicated that it expects half of those women to be chosen.

Latino leaders, one of whom proclaimed at Barry's victory party last month that "they owe us," were scheduled to meet over the weekend to come up with an agenda. Even those groups that jumped on the victorybound Barry political bandwagon late, such as leaders of organized labor, also are beginning to stake their claims in the coming administration.

And there are the stalwart campaign workers such as Betty King, who now sits confidently at her desk in the Barry transition office near a white button bearing the letters, "WBBTP" -- With Barry Before The Primary.

"It's like veteran's preference," King said, talking about the button's message and her chances of getting one of the coveted top-level jobs in the Barry administration. "If there are three people who have identical qualifications, the veteran ought to get the preference. I'm the veteran."

Delano E. Lewis, chairman of the Barry transition team, says he is sure of two things.

One is that since this is the first passing of the reins of District government from one elected administration to another in this century, no one knows for sure how many patronage jobs there are to be filled.

Of 45,000 jobs in city government, only about 100 have been identified so far that could be filed immediately with Barry appointees.

The second thing Lewis said he is sure of, however, is that there are not enough positions to go around. "There is a problem because everybody wants some immediate job around the mayor," Lewis said. "What Marion has to do is to do the best he can to be responsive. He's actually not going to be able to please everyone."

The same kind of limitations are likely to apply to the various agendas being formulated. When one key transition official was told, for example, that gay groups want Department of Human Resources employes to be prohibited from discouraging apparently gay children from being homosexuals, his response was an incredulous shaking of the head.

"That won't fly," the official said privately, "That WON'T fly."

So far, the transition team has placed most of its emphasis on developing a structure for the new administration, which takes office Jan. 2, and on making sure the new mayor knows which of the many inherited problems will have to be responded to at once -- such as answering ongoing court cases on time.

In the final four weeks before the inauguration, the focus is shifting more toward long-range policy planning and staffing of the administration, especially in the mayor's office. By the time the administration takes office, transition planners hope to have at least four top posts filled -- city administrator, housing director, corporation counsel and budget director.

A small task force headed by lawyer Herbert O. Reid has been set up to find a new corporation counsel. Selection of the other three is being done primarily by Barry, Lewis and two longtime Barry confidants -- Ivanhoe Donaldson and James O. Gibson.

Only two names have surfaced so far as condidates for the job of city administrator, the number-two post in District government.

One is acting city budget director Gladys W. Mack. The other, disclosed by transition officials last week, is Elijah B. Rogers, 39, a graduate of Howard University who once served as assistant city manager in Richmond and since 1976 has been city manager in Berkeley, Calif.

Rogers said in a telephone interview last week that he has not been contacted officially by Barry or anyone from the transition team, but indicated he would be interested in an offer to be city administrator of the District.

Top transition officials have already decided, sources said last week, that the new city administrator must be black, largely because Washington is a city with a 70 percent black population.

"If there're gonna be black city administrators any place in the United States, D.C. is gonna be one of those places," one major Barry adviser said privately.

Some of Barry's key campaign workers already have been tentatively selected for key jobs in the administration. Campaign manager Ivanhoe Donaldson probably will be general assistant to the mayor, sources indicated. Press secretary Florence Tate will be the mayor's press secretary. Fund raiser Ann Kinney is likely to work in the Office of Business and Economic Development, a key transition official said last week, and campaign scheduler Sybil Hammond is likely to have a similar job in the mayor's office.

All of these people have offices on the third floor of the National Building, where the transition team has its headquarters. But on the sixth floor, there are about a dozen other campaign loyalists, most of whom also expect to receive choice jobs working directly with the mayor.

It is among these people that there is more uncertainty about being able to get the jobs they want in the new administration.

The 32-point gay agenda put forth by a coalition of seven local groups is centered on changes in the D.C. Office of Human Rights, which enforces the city's antidiscrimination laws under which homosexuals and others are protected.

Among the major points on the agenda is a call for the removal of OHR director James W. Baldwin, who was not on Barry's original "hit list" of allegedly incompetent administrators targeted for removal. Key Barry aides said last week they still are not sure whether Barry will fire Baldwin.

The gay groups want to play a key role in the selection of a new director for the office and think that persons at all levels of government, especially cabinet members, should be screened for sensitivity to gay concerns.

"Mr. Barry routinely included such screening when he hired his council staff and we believe this is a practice worth continuing," the agenda reads.

The agenda, drawn up by a coalition of seven gay and feminist groups headed by the Gay Activists Alliance, also asks public financial support for some programs now receiving private funds, including the "Violence Against Gays Project" and a medical clinic for homosexuals.

Union leader Richardson said that while organized labor is still working on its proposals for the new administration, playing a major role in the selection of a new director of the city's department of labor is paramount.

The department's present acting director, Thomas A. Wilkins, is "completely unacceptable," Richardson said. Barry has already included Wilkins on his hit list.

Richardson also said the city's organized labor leaders expect to have someone in the new administration they can contact directly about their problems and from whom they can receive prompt and accurate service.

"It's got to either be somebody who's got the mayor's ear... or of sufficient rank to act on his own within guidelines set by the mayor," he said.