John Overton Eisinger Sr., 80, a Bethesda businessman since the mid-1940s, died Tuesday at a hospital in West Palm Beach, Fla., after a stroke. He maintained homes both there and in Bethesda.

He was the president of the Bethesda Builders Supply Co. and the Bethesda-Chevy Chase Land Co.

Mr. Eisinger was born in Washington. He was a descendant of Ninian Beall who was given a land grant in 1703 of property in Georgetown, which he called Rock of Dumbarton and which later became Dumbarton Oaks.

Mr. Eisinger served in the U.S. Navy in World War I. He then attended George Washington University where he received a degree in mechanical engineering.

After working several years for the National Bureau of Standards, he joined the Standard Oil Co. of Indiana in about 1924 and remained with that company for 20 years, reaching the position of research director.

Mr. Eisinger had been active in St. John's Episcopal Church in Chevy Chase, the Chevy Chase Rotary Club and the Congressional and Kenwood Country Clubs.

He is survived by his wife, the former Helen Nixon, of the homes; a son, John O. Jr., of Bethesda; a sister, Octavia E. Webb, of Chevy Chase, and a brother, Constantine, of Bethesda.