Virginia Spence Lescure, 80, a native Washingtonian and a founder of the Jamestowne Society of Northern Virginia, died of cardiac arrest Sunday at Sibley Memorial Hospital.

Mrs. Lescure was a descendent of the Lords Baltimore, proprietors of Colonial Maryland, and the Spence family, which settled in Worcester County, Md., in 1680. She graduated from old Western High School here and the University of Maryland, where her late father, Prof. Thomas H. Spence, was dean emeritus. The university was founded by her great-grandfather, Charles B. Calvert.

She was the widow of William J. Lescure, a lawyer from Harrisburg, Pa. After his death in 1954, she moved to Washington, where she became active in the Episcopal dioceses of Washington and Maryland. She was a member of St. Margaret's Episcopal Church here.

Mrs. Lescure also was active in the Junior League of Washington. She was a member of the Sulgrave Club, Colonial Dames of America, the Descendants of the Lords of the Maryland Manors and the Ark and Dove Society.

Survivors include a son, William J. Lescure III of Bethesda; two sisters, Charlotte Wilton of Alexandria, and Mary Spence of Washington and three grandchildren.

The family suggests that expressions of sympathy be in the form of contributions to St. Margaret's Episcopal Church here.