John O. Eisinger Jr., 49, a Bethesda businessman and prize-winning amateur golfer, died Saturday at Washington Adventist Hospital of complications following abdominal surgery. He lived in Bethesda.

A native of Chicago, Mr. Eisinger graduated from Bethesda-Chevy Chase High School. While attending Duke University, he beat Arnold Palmer in a Duke-Wade Forest golf match held in Durham, N.C. Palmer, was captain of the Wake Forest team, was considered unbeatable at the time and Mr. Eisinger was said to be the only golfer to defeat Palmer during his callege career.

After serving in the Army for tow years, Mr. Eisinger toured on the professinal golf circuit as an amateur. He then joined his family's business, Bethesda Builders Supply Inc. in Bethesda.

Mr. Eisinger won the Kenwood Country Club Championship in 1955 and seven Congressional Country Club championships from the mid-1950s to the mid-1960s. He also competed in several national amateur golf championships and the U.S. Open at Denver in 1960. As a junior golfer, he won junior club championships at Kenwood in 1947, 1948 and 1952.

In 1962, he married the former Barbara Anne Babcock, of Medford, Mass., who survives. He is also survived by his mother, Helen Nixon Eisinger of West Palm Beach, Fla.