John R. Neale, 69, a retired senior vice president of the American Security Corporation who was active in real estate financing here for nearly 45 years, died of a heart ailment Wednesday at Suburban Hospital. He lived in Bethesda and Ocean City, Md.

Mr. Neale began his career with American Security & Trust Company, of which the American Security Corpration is an affiliate, as a runner in 1930. He transferred to the bank's real estate department later that year. He became an assistant vice president in 1948, a vice president two years later and the vice president in charge of real estate in 1955.

In 1968, Mr. Neale was elected to the newly created post of senior vice president of the American Security Corporation, a position he held until retiring in 1976. He also served on the bank's board of directors.

Mr. Neale was a member of the boards of the Real Estate Title Insurance Corporation, the B.F. Saul Company and Chevy Chase Savings & Loan Association. He was a trustee of the B.F. Saul Investment Trust.

He also was a member of the Washington Board of Realtors, the Metropolitan Washington Board of Trade and the Washington chapter of the Mortgage Bankers Association.

Mr. Neale was a native of Washington and graduate of the old Central High School. He attended George Washington University and was a 1934 graduate of Columbus University Law School. He served with the Army Air Forces in Europe during World War II.

Survivors include his wife, Sara (Dolly) Neale, whom he married in 1939, of Bethesda and Ocean City; a daughter, Joan N. Spermo of Bethesda; two sons, John R. Jr. of Potomac and Joseph H. of Bethesda; two brothers, James F. Jr. of Williamsville, N.Y., and Joseph M. of Mount Jackson, Va., and five grandchildren.

The family suggests that expressions of sympathy be in the form of contributions to the Children's Hospital National Medical Center, Washington, D.C.