John O. Perpener, 68, associate dean of the University of the District of Columbia who had been associate vice president for academic affairs of the old Washington Technical Institute, died Jan. 29 at Fairfax Hospital following a stroke.

Dr. Perpener, who lived in Washington, was born in Fort Worth. He served in the Navy in the Pacific in World War II. He graduated from Texas College in Tyler, Tex., and received master's and doctoral degrees in education from the University of Colorado.

He was dean of instruction at Lane College in Jackson, Tenn., and in 1959 went to Jarvis Christian College in Hawkins, Tex. He was president there when he resigned in 1971 to join the Washington Technical Institute as associate vice president for academic affairs. He moved to UDC when it was formed with WTI as part of it.

Dr. Perpener was a member of the National Education Association, the American Association of Higher Education and the Twelfth Street Christian Church.

Survivors include his wife, Maye E. Perpener of Washington; two children, John O. III, of Washington, and Carmena Richards of St. Thomas, V.I.; two sisters, Winifred Sherman of Hawkins, and Sadie Phillips of Neches, Tex.; one brother, Andrew Perpener of Fort Worth, and one grandchild.