C. Lester Trotter, 69, a retired assistant director of the FBI who was in charge of the identification division, died Aug. 13 at Humana Hospital in St. Petersburg, Fla. He had kidney and liver ailments.

Mr. Trotter, who lived in Treasure Island, Fla., was born in Bryantown in Charles County, Md. He grew up in Washington and graduated from Eastern High School.

He began his career at the FBI in 1934. While working, he went to night classes at Columbus University and earned a law degree. In 1940, he was appointed a special agent in the FBI.

Mr. Trotter was assigned to FBI field offices in Salt Lake City and New York City and then to the Washington field office. Promoted to inspector, he was put in charge of the records division at FBI headquarters. His next assignment was supervising the agency's training programs.

In 1956, he was made chief of the identification division and later that year was named an assistant director. He retired in 1969.

The next year, Mr. Trotter moved to Florida from his home in Forest Heights, Md. From 1972 to 1979, he was a special investigator with the Florida State Attorney's Office for Pinellas and Pasco counties.

Mr. Trotter was a member of the D.C. Bar and the Society of Former Special Agents of the FBI.

Survivors include his wife of 40 years, Lee Trotter of Treasure Island; three sons, Charles L. Jr., of Upper Marlboro, Gregory, of Kennesaw, Ga., and Mark, of Safety Harbor, Fla.; one daughter, Suzanne Jones of Rosedale, Ind.; two brothers, John, of Upper Marlboro, and Richard, of Washington; and three grandchildren.