The Williams sisters will play each other in the Australian Open only if both make the final.
Serena Williams is seeded first and will open her bid for a fourth straight Grand Slam title against Emilie Loit of France. Venus Williams is seeded second and will begin against Russia's Svetlana Kuznetsova.
Among the men, Lleyton Hewitt faces a possible third-round match with former No. 1 Gustavo Kuerten. Hewitt, the top-seeded Aussie, was paired today against a qualifier in the first round and American Cecil Mamiit or another qualifier in the second round before a possible showdown with Kuerten.
The Williams sisters have met in the last three Grand Slam finals and are on different sides of the draw at Melbourne Park for the first time.
Serena could meet Els Callens of Belgium or Martina Sucha of Slovakia in the second round. Venus's first seeded opponent could be No. 27 Lisa Raymond.
No. 4 Kim Clijsters of Belgium, four-time Australian champion Monica Seles and 1995 winner Mary Pierce were in Serena's half of the draw. Two-time defending champion Jennifer Capriati, seeded third, was in the same half of the draw as Venus.
Capriati starts her second consecutive defense in Melbourne against Germany's Merlene Weingartner and could meet No. 5 Justine Henin or No. 9 Lindsay Davenport, the Australian Open champ in 2000, in the quarterfinals.
Hewitt could meet Andy Roddick or Jiri Novak in the quarterfinals and third-seeded Marat Safin in the semis.
Second-seeded Andre Agassi, who has three Australian championships among his seven Grand Slam titles, will open against Brian Vahaly, an American who jumped 306 places in the rankings to finish inside the top 100 in 2002.
Agassi's second-round opponent would be South Korea's Lee Hyung Taik or Spain's David Ferrer. Agassi was seeded to meet Frenchman Nicolas Escude in the third round.
Agassi, 32, could face Argentina's Guillermo Canas or Russia's Yevgeny Kafelnikov in the fourth round.
Agassi had a wrist injury last year and couldn't defend his Australian Open title. He was the losing finalist to Pete Sampras at the U.S. Open in the last Grand Slam. Sampras won't be playing in Australia.