Loudoun County Democrats last week called for Supervisor Eugene A. Delgaudio to resign from public office, after accusations by a former county aide who said the supervisor used county resources for his political campaign and subjected employees to a hostile work environment.

Hours after the aide’s allegations were reported by The Washington Post, the Loudoun County Democratic Committee issued a statement Wednesday declaring that Delgaudio (R-Sterling) should step down from the Board of Supervisors.

“He has proved himself unfit for office many times over, but these accusations remove any doubt,” the statement said. “His misuse of office, treatment of staff, and flagrant violation of county policies are unacceptable.”

Donna Mateer, the former aide who filed a statement with the county Human Resources Department detailing her concerns after she was fired in March, said she made hundreds of phone calls to schedule meetings with potential donors. Three people who agreed to meet with Delgaudio said the supervisor solicited donations to his political campaign.

County employees, including board aides, are prohibited from engaging in political activity during work hours.

Local Democrats have called for Delgaudio’s resignation in the past, including earlier in the year, after his nonprofit organization was officially designated as a hate group by the Southern Poverty Law Center.

The statement last week also urged supervisors Chairman Scott K. York (R-At Large), along with the other county supervisors and Republican leaders, to “decry Delgaudio’s actions.”

Neither York nor the Loudoun County Republican Committee responded to requests for comment.

Al Nevarez, Delgaudio’s Democratic opponent in last November’s election, said in the Democratic Committee’s statement that Delgaudio “owes the people of Sterling an explanation” for allegedly using public resources to conduct campaign business.

Nevarez also voiced concern about Mateer’s claim that Delgaudio told her to report to the office manager at Public Advocate of the United States, a nonprofit group led by Delgaudio since 1981 that was sued by the Southern Poverty Law Center in federal court Wednesday.

“It’s time for Eugene Delgaudio to come clean with the people of Sterling and resign immediately,” Nevarez said in the statement.

On Thursday, Delgaudio posted a response to The Post’s report on his campaign Web site, www.joineugene.com, reasserting his claim that the fundraising project assigned to Mateer was strictly to benefit the Lower Loudoun Boys Football League.