Laura Bush's spokeswoman said Wednesday that the former first lady did not approve the use of her 2010 comments about same-sex marriage in an ad by the Respect for Marriage Coalition, and has asked that the footage be removed.

“When [Mrs. Bush] became aware of the advertisement last night, we requested that the group remove her from it,” Anne McDonald told the Dallas Morning News in a statement.

The coalition, which is co-chaired by Freedom to Marry and the Human Rights Campaign,  listed Bush, Colin Powell and former vice president Dick Cheney among the political figures featured in the ad campaign. The first ads aired Wednesday, and the group said they would be in heavy rotation on cable news and Sunday news shows in the coming weeks.

The clip of Bush is from a 2010 interview on "Larry King Live," in which she discussed her and her husband's differing views on same-sex marriage. She called opposition to marriage equality a "generational thing."

"I think that we ought to definitely look at it and debate it," she said then. "I think there are a lot of people who have trouble coming to terms because they see marriage as traditionally between a man and a woman, but I also know when couples are committed to each other and love each other, they ought to have, I think, the same sort of rights that everyone has."

Update: The Respect for Marriage Coalition will remove the ads featuring Laura Bush  starting this weekend and  replace them with the ad below featuring a Republican military veteran.

"We used public comments for this ad from American leaders who have expressed support for civil marriage," a spokesperson for the coalition said Thursday. "We appreciate Mrs. Bush’s previous comments but are sorry she didn’t want to be included in an ad.  The ad launched a public education campaign that will now move to new and different voices that reflect the depth and breadth of our support."