"America needs principled leaders like Cory Gardner in the Senate," Rubio says in the English-language ad. "That's how we'll end gridlock."
There are many Hispanic residents in Colorado -- more than one in five, according to the U.S. Census -- which is why Rubio's ad appears in Spanish as well as English.
Gardner is running against Sen. Mark Udall (D) in a race early polls show is very competitive.
The Chamber also released an ad supporting Senate Minority Leader Mitch McConnell (R-Ky.) ahead of his May 20 primary against businessman Matt Bevin. Reid Wilson has the rest of the details on the group's new wave of ads in today's Read In:
The Chamber will start running 13 new ads today on behalf of Senate and House Republicans in what spokeswoman Blair Holmes described as a "massive campaign." The chamber on Tuesday dropped almost $660,000 on broadcast and cable ads in the Denver, Colorado Springs and Grand Junction markets on behalf of Senate candidate Cory Gardner. They'll feature a small business owner in a new ad for Senate Minority Leader Mitch McConnell, too.-- The Chamber is spending on behalf of former Rep. Doug Ose (R-Calif.), Rep. David Valadao (R-Calif.), Rep. Mike Coffman (R-Colo.), Rep. Mike Simpson (R-Idaho), former Rep. Bob Dold (R-Ill.), Rep. Andy Barr (R-Ky.), former Massachusetts state Sen. Richard Tisei (R), Rep. Dan Benishek (R-Mich.), businessman Stuart Mills in Minnesota's 8th District, Rep. Joe Heck (R-Nev.) and Rep. Chris Gibson (R-N.Y.).-- Total spent on House candidates in this ad flight through the end of May: A little north of $1.1 million. The Chamber has spent about $6.6 million since the beginning of February.