He then asked rallygoers to raise their hands if they were Christian conservatives — before asking non-Christian conservatives to do so as well, and a few raised their hands.
The moment drew immediate scorn from critics.
“Raise your hand, Christian conservatives, everybody. Raise your hand if you're not a Christian conservative. I want to see this, right. Oh, there's a couple people, that's all right,” Trump said smiling, waving his hand in the air. “I think we’ll keep them. Should we keep them in the room? Yes? I think so.”
The Council on American-Islamic Relations blasted Trump on Wednesday evening.
“Given Mr. Trump’s history of targeting religious and ethnic minorities, it is disturbing that he would single out non-Christians during his rally in Iowa. It is clear that his vision of America is one of division and exclusion, not unity and acceptance," said CAIR spokesman Ibrahim Hooper in a statement.
This story has been updated.