To supporters of Israel and many others, it was a remarkably powerful moment, showing how the world was ignoring the risks posed by a nuclear deal with Iran. To critics of the sometimes divisive Netanyahu, it was laughable.
Online, a variety of mocking versions of the speech have begun to circulate. One YouTube user uploaded a video of the silence, with the interlude filled with "The Sound of Silence" by Simon and Garfunkel.
Another added the theme music from the Larry David sitcom "Curb Your Enthusiasm."
A song by Major Lazer, "Pun de floor," also filled the silence.
And there were plenty of other suggestions.
Netanyahu's fiery speeches have become something of a tradition at the United Nations. Often, these speeches have involved "props" to designed to lend a rhetorical heft – in 2012, for example, the Israeli leader brought a diagram of a bomb on stage, a move which also prompted online ridicule. Last year, Netanyahu brought photographs that he said showed children playing near a rocket launcher operated by the Palestinian group Hamas.
Following weighty references to the Holocaust and Iran's threats against Israel, Netanyahu's silence had been intended to hammer home a point. However, judging from the response, much of the online audience has already accepted the Iran deal and moved on.